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Fig. 2. Rescue of Moe^PL54 lethality. (A) Confocal image of 50% pd hs>Moesin Myc retina shows F-actin staining (red) and full-length wild-type Moesin (anti-Myc, green). Myc-tagged Moesin concentrates at the rhabdomere base and is distributed throughout the cell cytoplasm (asterisks). Gal4/UAS transgenes show variable levels of protein expression in pupal eyes. (B) Confocal micrograph of a live 65% pd hs>Moesin GFP retina. Strong GFP signal is seen in rhabdomeres and pigment cell cytoplasm; lesser signal is also evident in photoreceptor cytoplasm. (C) Act5C>Moe-GFP rescues Moe^PL54 lethality. Confocal image of 70% pd retina shows F-actin staining (red) and Moesin-GFP (green) in the Moe^PL54 hemizygous background. Photoreceptors with the most severe defects show the lowest detectable levels of Moesin-GFP (arrowheads). Moesin-GFP concentrates at the rhabdomere base in cells expressing lower protein amounts (arrow). (D) Electron micrograph cross-section of a 1-day post-eclosion Moe^PL54/Y; Act5C>Moe-GFP adult ommatidium. Photoreceptors of Act5C>Moesin-GFP rescued Moe^PL54 contain imperfect rhabdomeres, frequently exhibiting defects in size and microvillar organization. Scale bars: 10 µm in A,B,C; 1 µm in D.

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