|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Moesin RNAi disorganizes F-actin and apical microvilli. (A, C) Confocal
micrographs show F-actin staining (red) and Moesin immunolocalization (green).
(A) In a 57% pd hs>IR-Moesin retina, photoreceptors lacking Moesin
(arrowheads) are reduced in size and exhibit abnormal F-actin distribution.
The asterisked ommatidium (enlarged, right) shows lack of Moesin in R1, R3 and
R7. (B) An electron micrograph of 57% pd hs>IR-Moesin ommatidium
shows loosely organized rhabdomere microvilli (compare with
Fig. 5A); the rhabdomere of R5
is reduced. (C) GMR>IR-Moesin photoreceptor apical membrane
organization is devastated by Moesin loss. A single photoreceptor (arrowhead)
retains strong Moesin signal and an intact rhabdomere. (D) Electron micrograph
of 70% pd GMR>IR-Moesin ommatidium shows severe rhabdomere
defects. The asterisked photoreceptor lacks a rhabdomere. Adherens junctions
appear normal (B,D; arrows). Scale bars: 10 µm in A,C; 1 µm in B,D.
|
