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Fig. 5. Dominant-active T559D Moesin expression prevents photoreceptor apical
regionalization. (A,B) Electron micrographs of 65% pd wild type (A) and
hs>T559D Moesin Myc (B) ommatidium. The apical membrane, spanning
between adherens junctions (A,B; arrows), is severely disorganized by T559D
Moesin expression (B). In this ommatidium (B), R5 is less affected, showing
clear rhabdomere and stalk domains, presumably due to lower levels of T559D
Moesin expression. (C,D) The apical actin cytoskeleton is severely disrupted
in dominant-active Moesin-expressing cells, and Crumbs (C) does not properly
relocalize to the stalk. (D) The field in C, showing Crumbs staining (blue)
merged with staining for F-actin (red) and Myc (green). Red rhabdomeres of
`escaper' cells, not expressing T559D Moesin Myc, appear normal. (E)
The ommatidium marked with an asterisk in D is shown at a higher
magnification. T559D Moesin was not expressed equally in all cells in this
ommatidium, as reflected by the lack of Myc staining in the asterisked R7.
F-actin in the rhabdomere of R7 is orderly, and Crumbs was properly cleared
from the rhabdomere membrane and localizes to the stalk. Compare with
neighboring photoreceptors in D. Scale bars: 1 µm in A,B; 10 µm in
D.
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