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Fig. 4. Cuticle preparations of wild-type and T-Sp8 RNAi adult legs and antennae (proximal points to the left, distal to the right). (A) The adult leg is composed of the same leg segments as the embryo except that the tibia (Ti) and the tarsal segments (Ta) are distinct. (B) T-Sp8 RNAi weak phenotype. The leg lacks the tarsus. The pretarsal claw (Ptc) together with the club shaped tibia-like structure (arrowhead) is fused to a shortened femur. (C) T-Sp8 RNAi strong leg phenotype. Only the pretarsal claw (Ptc) is connected with a joint to the otherwise jointless leg stump. (D) In the wild-type antenna, six segments (dots) form the middle region between pedicel (Pd) and the club (Cl). (E) Adult T-Sp8 RNAi antennae frequently show a reduction in the number of middle segments. In this example, four segments (dots) can be seen. Cx, coxa; Tr, trochanter; Fe, femur.





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