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Fig. 5. Distal-less and dachshund expression in wild-type- and in TSp8 RNAi embryonic legs. The pleuropodium (pl) is nonspecifically stained in A and C. (A) Wild-type embryo at the end of the segmentation process. The long outstretched legs show Distal-less expression as a proximal `ring' (arrowhead) and a distal positioned `sock' (arrow). (B) In very strongly affected T-Sp8^RNAi legs, Distalless expression can be seen at the tip of the dramatically shortened leg (arrow). At the base of such a leg a dot of Distal-less expression (arrowhead) represents the rest of the `ring' domain. (C) The dachshund gene is expressed in three domains in wild-type embryonic legs: as a spot (S) near the body wall, weakly in a proximal (P) and stronger in a distal (D) domain. (D) All three domains appear to be present in T-Sp8^RNAi legs. The distal domain (D) is reduced in width. The distal leg tip as in the wild type is free of dachshund expression. The embryos shown in B and D most probably represents the very strong phenotype shown in Fig. 3D.

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