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Fig. 3. An EMS screening approach identifies novel mutations, which display severe
defects during visceral mesoderm development. The development of the visceral
mesoderm is visualised by Fas3 expression. (A-C) Wild-type development at
stages 11 (A), 12 (B) and stage 15 (C, arrowhead shows the midgut encircled by
the visceral mesoderm). (D-F) Examples for the different subgroups of
mutations are shown. (D) In embryos of the first subgroup (G1), no visceral
mesoderm can be detected at stage 11 (arrowhead). (E) In the second group
(G2), embryos develop the initial patches of the circular visceral muscles,
but these patches subsequently fail to form a continuous band (arrowhead). (F)
In the third group (G3), the continuous band is formed but the cells of the
visceral mesoderm do not migrate dorsally and ventrally to encircle the entire
midgut (arrowhead in F compare with arrowhead in C).
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