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Fig. 5. The fusion-competent myoblasts of the somatic mesoderm do not differentiate
in jebweli and
miliAlk mutants. (A-C) sns in situ
hybridisation of stage 11 embryos marks fcms in the visceral and somatic
mesoderm. In wild-type embryos, sns is expressed in two bands along
the entire length of the embryo (A). These bands are connected in a
ladder-like pattern, where the ventral band represents the fcms of the somatic
mesoderm (arrow in A), and the dorsal band consists of the fcms of the
visceral mesoderm (arrowhead in A). In jebweli
(B) and miliAlk (C) mutant embryos, the fcms of
the visceral mesoderm and some cells that connect the two bands are present
(arrowheads); however, the ventral band of fcms of the somatic mesoderm shows
no sns expression. (D-F) Alk (green) and Fas3 expression (red) in
stage 11 wild-type embryos. In addition to expression in the visceral
mesoderm, Alk is also transiently expressed in some cells in the neuroectoderm
and in patches in the somatic mesoderm (arrows in D,F). (G-I) Lmd expression
of stage 12 embryos. In the wild type, Lmd is expressed in two bands in the
fcms of the somatic and visceral mesoderm (G). In
jebweli (H) and
miliAlk (I) mutants, expression in both
mesodermal cell types can be observed.
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