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Fig. 1. el and noc expression in presumptive appendages. (A) Genomic organization of the elbow and no ocelli loci. The el transcript consists of three exons spanning 3.3 kb [EST clone RE67722 (Dorfman et al., 2002)]. noc is located 83 kb downstream of el and consists of two exons. The el¹ deletion removed 25 kb of DNA between the two coding regions and specifically affects expression of both genes in the wing imaginal disc, suggesting that it removes a common regulatory element shared by the two loci. el and noc were coexpressed at all stages examined. (B) Diagram of the proximal-distal subdivision of both wing and leg primordia by signaling molecules. In the leg imaginal disc, the combined activity of Dpp and Wg induces the expression of Dll in the presumptive appendage primordium (blue) and restricts the expression of Hth to the periphery of the disc, which will give rise to the adult body wall (red). In the wing imaginal disc, Wg activity induces the expression of Nub in the wing primordium (blue) and Vein (Vn) activity induces the expression of Hth in the periphery of the disc, which will give rise to the adult body wall (red). (C) Second instar leg disc labeled with antibodies to visualize Dll protein (blue), El protein (green) and Hth protein (red). Noc expression was identical to El expression (not shown). (D) Second instar wing disc labeled to visualize noc^Gal4 using UAS-GFP (green), Nub protein (blue) and Hth protein (red). Note that the expression domain of Noc is slightly broader than the domain of Nub and overlaps Hth expression. Hth repression lags behind Tsh repression and thus underestimates the size of the wing field at early stages (Wu and Cohen, 2002). (E) Mature third instar leg imaginal disc labeled as in C. Note the change in the relative expression pattern of El and Dll with respect to the earlier stage in C. (F) Mature third instar wing disc labeled as in (D). El and Noc are expressed in a ring corresponding to the wing hinge and in wedge shaped domains centered on the dorsal-ventral boundary.

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