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Fig. 3. Early el noc double mutant clones in the wing. (A,C) Wing discs with clones of cells lacking elbow and no ocelli (el3.3.1 noc{Delta}64) at an early stage. Nub protein is shown in green; Tsh protein in red. Clones are marked by the absence of ß-gal (blue). (A) Mutant clones (asterisks) sorted out from the Nub-expressing wing pouch. Twin clones (arrowheads) remain in the Nub-expressing domain. (B) Summary of the relative positions of clones of cells lacking el and noc (white circles) and their twins (black circles) when born in the wing primordium (green) or in the body wall (red). (C) Wing disc with a large el3.3.1 noc{Delta}64 double mutant clone in the wing pouch. Tsh (red) was ectopically expressed and Nub (green) was lost in the clone. Comparable clones also showed ectopic Hth expression (not shown). (D) Wing disc with large Minute+ el3.3.1 noc{Delta}64 double mutant clones. Asterisks mark clones abutting the wing pouch and remaining in the body wall region. Hth protein is shown in red. (E) Summary of the location of Minute+ el3.3.1 noc{Delta}64 double mutant clones in the body wall region of wing imaginal discs.





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