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Fig. 5. Early el noc double mutant clones in the leg. (A) Schematic representation of a leg showing the distribution of Minute[+] el^3.3.1 noc64 double mutant clones induced early in development (60 h AEL). Co, Coxa; Tr, Trochanter; Fe, Femur; Ti, Tibia; Ta, tarsal segments. (B) Summary of the relative position of clones of cells lacking el and no ocelli (grey circles) and their twins (black circles) when born in the proximal or distal leg. ta, tarsal segments. (C) Leg disc with Minute[+] el^3.3.1 noc⁶⁴ double mutant clones. Mutant cells were found in femur and tibia, but not in the tarsal segments (ta). Hth protein (red). Clones are marked by the absence of ßGal (blue). (D) Summary of the locations of Minute⁺ el^3.3.1 noc64 double mutant clones in the proximal region of leg imaginal discs. The position of each clone is depicted as one semi-transparent layer of grey. The darker an area is, the more clones were found in that region. (E) Leg disc with an el^3.3.1 noc64 double mutant clone induced early in development (60 hours AEL). Hth (red) was ectopically expressed in some clones (arrow). Note that the twin spot cannot be seen in this picture because the el^3.3.1 noc64 double mutant clones sort out from the epithelium and are therefore found in a different focal plane. Clones induced earlier were not recovered.

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