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Fig. 1. The OBLS forms ectopically at the lateral part of the telencephalon of
Pax6 mutants. (A-D) Lateral views and (E-L) coronal sections of
wild-type (WT, A,C,E,F,I,J) and Pax6-mutant telencephalons (-/-,
B,D,G,H,K,L), stained with anti-neruopilin 1 (Nrp, A,B,I,K), anti-Calretinin
(CR, I,K), anti-GAD67 antibodies (J,L) or hybridized with specific probes for
neuropilin 1 (Nrp, E,G), netrin G1 (C,D,F,H). A-H and I-L show
E16.5 and E18.5 embryos, respectively. Nrp and netrin G1 are expressed in the
protruding OB in the wild type (arrowheads in A,C), whereas these markers are
observed at the lateral part of the mutant telencephalon (arrowheads in B,D).
Note that Nrp protein is localized mostly at the neuropiles and axons of
mitral cells (arrowheads and arrows in A,B). The distribution of Nrp
and netrin G1 overlap in the OB and the OBLS (E-H). Nrp, CR and GAD67 are
expressed in the different types of neurons of the OB and the OBLS (I-L). egl,
external granule cell layer; epl, external plexiform layer; grl, glomerular
layer; mcl, mitral cell layer. Scale bars: A-D, 1 mm; E-L, 50 µm.
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