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Fig. 4. Misrouting of mitral cell-precursors in the telencephalon of Pax6 mutants. (A-C) Lateral view (A) and parasagittal sections (B,C) of the head of electroporated, mutant embryos. At 24 hours after electroporation, there is GFP fluorescence in the rostral-most telencephalon. (D-D'',E-E'') Distribution of GFP-positive cells following TOC for 3 days. E-E'' are transverse sections of a TOC sample. GFP-positive cells migrate caudally (arrowheads in D-D''), and constitute the OBLS, which is marked by CR and Nrp immunostaining (arrowheads in D',E'). (F-F'') Confocal images of GFP-positive cells in the OBLS. Nrp protein is localized at the neuropiles of GFP-positive cells (arrowheads). (G-G'') GFP-positive cells are also labeled with BrdU (arrowheads). tel, telencephalon; LV, lateral ventricle. Scale bars: A-C, 500 µm; D-E'', 100 µm; F-G'', 10 µm.





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