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Fig. 5. Misrouting is caused by non-cell autonomous defects for the mitral cell
precursors. (A) Experimental procedures for cell transplantation. The rostral
part of the telencephalon of donor embryos was isolated, and neuroepithelial
cells dissociated with enzymes and transplanted into the rostral part of the
host telencephalon. Donor cells were isolated from GFP-transgenic rat embryos.
(B,C) Distribution pattern of cells derived from homozygous mutants (-/-)
transplanted into heterozygous (+/) embryos after 48 hours of WEC followed by
TOC. Donor cells contribute to the host OB and express Nrp (C,C'). (D,E)
Distribution pattern of +/- derived cells in -/- embryos after 48 hours in WEC
(D) and 2 days in TOC (E). Donor cells migrate caudally toward the lateral
telencephalon and invade the OBLS, which is marked by Nrp immunostaining (E).
A donor-derived cell in the OBLS expresses Nrp (E'). (F) The
distribution of donor cells after transplantation. The white bar indicates
that donor cells are located only at the rostral part of the telencephalon.
Black bars indicate that donor cells are located in both rostral and lateral
parts of the host telencephalon. Scale bars: B,D, 500 µm; C,E, 100 µm;
C',E', 10 µm.
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