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Fig. 6. Misrouting of the mitral cell precursors in Pax6 mutants is not caused by lack of olfactory nerve innervation. (A) Experimental procedures for brain culture. After removal of the olfactory epithelium and head mesenchyme, brain tubes were cultured in rotating bottles (whole brain culture, WBC) and in TOC for 2 days. (B,C) Distribution of netrin G1-positive cells in the wild-type (WT; B) and the mutant (-/-; C) telencephalon after WBC. In the wild-type telencephalon, netrin G1-positive cells are located in the rostral-most telencephalon (arrowhead in B), whereas in the mutant they are in the lateral part of the telencephalon (arrowhead in C). Scale bar: 500 µm.





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