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Fig. 4. Hex is not required for expression of early pancreatic genes in
vitro. (A,B) RT-PCR analysis of E9.5 dorsal and ventral midgut tissues and of
ventral endoderm explants isolated from E8.25 (2-6S) embryos and cultured for
48 hours. Different cycle steps are shown; albumin and actin PCR was performed
in the same PCR reaction (Gualdi et al.,
1996). PCR cycle numbers for other markers were normalized by
actin levels. Positive controls were embryonic liver for albumin and E13.5
embryonic RNA for the other genes. (C) Representative ventral endoderm
cultures used for the RT-PCR shown in B. (D) RT-PCR analysis of ventral
endoderm explants isolated from E8.25 (2-6S) embryos and cultured for 48 hours
with or without cardiogenic mesoderm; a headfold ectoderm explant served as a
negative control.
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