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Fig. 1. Identification of minimal cis-acting neural crest enhancer regions in the proximal Pax3 genomic region. Constructs 1-10 used for the creation of transgenic mice (not to scale, see Materials and methods for construct details). Neural crest enhancer elements, NCE1 and NCE2, are in red. The Tead binding site within NCE2 is indicated and the mutant version of this site used in subsequent experiments is shown. The number of genotype positive E10.5-11.5 embryos obtained from injection of each construct is shown and those expressing ß-galactosidase are indicated.





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