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Fig. 5. Expression of SYCP3, RAD51 and ${gamma}$ -H2AX in Mili^-/- spermatocytes. Anti-SYCP3 immunostaining (A-D), anti-RAD51 staining (E,H), anti- ${gamma}$ -H2AX staining (K,N) and DAPI staining (F,I,L,O), and the merged images of RAD51 or ${gamma}$ -H2AX and DAPI (G,J,M,P) in Mili^+/- (upper panels of each pair) and Mili^-/- (lower panels of each pare) testes. Arrows show the ${gamma}$ -H2AX restricted to the sex bodies in early pachytene spermatocyte. All images except A and C were observed by confocal microscopy. Scale bars: 10 µm.

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