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Fig. 2. XSeb4R is strongly expressed in the developing central nervous
system. (A) Embryo (stage 10.5) presented in a posterior view, with dorsal up,
shows a ring-like expression around the blastopore. (B) Transverse section (as
indicated in A) showing Xseb4R in the mesoderm (me). (C) Embryo
(stage 14) presented dorsally, with anterior up, revealing three bilateral
stripes, medial (m), intermediate (i) and lateral (l), of Xseb4R
expression in the open neural plate, as well as expression in the trigeminal
placode (tp) and in the presumptive ventral midbrain/forebrain (vmfb) area.
(D) Transverse section (as indicated in C) showing Xseb4R signals in
both the sensorial layer (sl) of the neuro-ectoderm and in the mesoderm (me)
as well as the notochord (no). (E,F) Embryos (stage 17 and 20, respectively)
presented dorsally, with anterior up, showing additional Xseb4R
expression in the olfactory placode (op), eye (e), forebrain (fb), midbrain
(mb), hindbrain (hb) and spinal cord (sc). (G,H) Embryos (stage 24 and 32,
respectively) placed laterally with anterior left showing Xseb4R
expression in the tail bud (tb) and tail tip (tt). (I) Transverse section (as
indicated in H) showing Xseb4R signal in the subventricular zone
(svz) of the neural tube. (J) Lateral view showing a weak expression of
Xseb4R expression in the developing pronephros (pn). (K) Two
bilateral stripes of Xseb4R expression in cells associated with blood
islands (bi), and a strong signal in the liver (li). (L) Expression during the
characteristic Y-shape formation of blood islands. (M)
Xseb4R-expressing cells in the area associated with duodenum (du)
formation. (N,O) Embryos presented in the ventral view showing pigmented eye
(pe) and Xseb4R expression in the presumptive rectum/anus (r/a).
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