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Fig. 6. Xseb4R is involved in the regulation of primary neurogenesis in
Xenopus embryos (A-C) Dorsal views of embryos injected with control
lacZ mRNA (A), 200 pg of Xseb4R mRNA plus lacZ mRNA
(B), or 50 pg of Xseb4R mRNA plus lacZ mRNA (C), and stained
for N-tubulin expression (dark blue). ß-galactosidase activity
is shown in light blue. Whereas the high dose (200 pg) of Xseb4R
represses N-tubulin expression on the injected side (arrow in B), the
low dose (50 pg) leads to the expansion of the N-tubulin domain
(arrow in C). (DG) Dorsal views of embryos after injection of the fusion
construct Xseb4R-GR. Induction with dexamethasone was performed
between stage 9.5 and 10 (D), or between stage 10.5 and 11 (E). F and G show
non-induced control embryos. Whereas induction of Xseb4RGR between
stage 9.5 and 10 represses N-tubulin expression in the injected side (arrow in
D), induction of Xseb4R-GR between stage 10.5 and 11 leads to
expansion of the N-tubulin domain (arrow in E). (H,I) Dorsal views of
embryos after injection of control Mo (H) or Xseb4R Mo1 (I).
Xseb4R Mo1 leads to a repression of N-tubulin expression in
the injected side (arrowheads in I). (J) Effects of various doses of control
Mo and Xseb4R Mo1 on N-tubulin expression. Injections of 10
or 20 ng of Xseb4R Mo1 lead to a decrease of N-tubulin
expression compared with control embryos.
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