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Fig. 5. Mutants that lack axial mesoderm specify PMNs normally. (A,B) Lateral views of cyc;flh mutant trunks. (C) Cross-section through the anterior trunk of cyc;flh mutant. (D) Lateral view of ntl single mutant trunk. (E) Lateral view and (F) cross-section of the anterior trunk of a ntl single mutant sibling from a ntl;spt cross. (A,D) Islet antibody + islet2 in situ hybridization at 18-21 hpf. Islet antibody staining is nuclear and brown; islet2 staining is blue and cytoplasmic. *Brown-only cell (MiP). Broken lines indicate somite boundaries. MiPs and CaPs are specified relatively normally in cyc;flh (A) and ntl (D) mutants. (B,C,E,F) znp1 antibody at 26-30 hpf. Ventral CaP axons are visible in all cases (black circle). MiP axons are visible in some whole mounts and in cross-sections (white arrow). Scale bar: 50 µm.





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