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Fig. 3. Specification of CGC is maintained in RL cultures independently of
Math1 expression. Rhombic lip cells from Math1+/+
(WT), Math1ß-gal/+ (Het) and
Math1ß-gal/ß-gal (Null) were
cultured and analyzed by RT-PCR with Zic1, Zipro1 and
ß-actin-specific primers after 3 and 6 days in vitro. The expression of
Zic1 and Zipro1 is constant in cultures from all genotypes
and along the culturing periods. + and - indicate the presence and absence of
reverse transcriptase, respectively.
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