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Fig. 4. Math1 is necessary for process outgrowth in rhombic lip cultured cells. Immunodetection of ß-tubulin (A-C), phosphorylated neurofilaments (D-F), 160 kDa neurofilament (G-I) and NCAM (J-L) in rhombic lip cells after 6 days in culture. The antibodies decorate process extensions from cells from Math1+/+ (WT, A,D,G,J) and Math1ß-gal/+ (Het, B,E,H,K), but not Math1ß-gal/ß-gal (Null, C,F,I,L). Counterstaining by DAPI (M-O) displays similar cell densities in all cultures. Scale bar: 50 µm.





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