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Fig. 9. Reduction of hyaluronic acid (HA) in the developing semicircular canals in dfna5 morphants (54 hpf larvae). (A,D) Outline of the structures in a dorsolateral view of wild-type (B,C) and dfna5 ATG-MO injected larval ears (E,F). The differential interference contrast (DIC) and corresponding fluorescent images are focussed at the level of the anterior column. An outgrowing protrusion of the anterior column (ac) in the wild-type ear (B) is filled with HA as shown by staining with biotinylated HA-binding protein (C). The lateral (lc) and posterior columns (pc) are out of focus in C. HA is reduced in both protrusions of the anterior column in a dfna5 ATG-MO injected larval ear (E,F). Scale bar: 35 mm.





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