DEV00966 Supplemental Figures

Files in this Data Supplement:

• Supplemental Figures Powerpoint -

  Fig. S1. Deletion of Foxa2 caused goblet cell differentiation. SP-C-rtTA, Foxa2^?/? (A,C) and CCSP-rtTA, Foxa2^?/? (B,D) transgenic mice were maintained on doxycycline from E0 and killed at PN16. Staining for Foxa2 demonstrated extensive loss of Foxa2 in small and large airways in the CCSP-rtTA, Foxa2^?/? mice. Although goblet cells lined most of the conducting airways in the CCSP-rtTA compound mice, Foxa2 targeting and goblet cell transformation were less extensive in the larger airways of SP-C-rtTA, Foxa2^?/? mice (C) and goblet cell differentiation was less extensive. Scale bar: 100 mm.

  Fig. S2. Western blot demonstrate decreased SP-B expression. SP-C compound mice and their littermate controls were maintained on doxycycline from E0. Lung homogenates were prepared at PN16. Equal amount of proteins were analyzed by western blot using SP-B or SP-C rabbit polyclonal antibodies. Protein quantity was analyzed by ImageQuant software. Control values were arbitrarily set to 100%. *P<0.05 compared with control mice (by Student’s t-test).

  Fig. S3. Immunohistochemistry for SP-B, CCSP, Pro-SP-C and TTF-1. Lung sections were prepared on E18.5. Triple transgene mice, SP-C-rtTA^-/tg, (tetO)₇CMV-Cre^-/tg, Foxa2^loxP/loxP and their littermate controls were treated with doxycycline from E0. Lung sections (E18.5) were stained for mature SP-B (A,B), CCSP (C,D), proSP‑C (E,F) and TTF-1 (G,H). Figures are representative of n=4. Scale bar: 100 mm.