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Fig. 6. Rescue of tws mutant phenotypes by UAS-Tws. (A) tws⁶⁰/tws^{60 pupa.} Homozygous tws^P and tws⁶⁰ mutants die at early pupal stages and do not show any signs of appendage development. (B,C) Ventral (B) and dorsal (C) views of two different tws⁶⁰/tws⁶⁰ pharate adults rescued by the expression of UAS-Tws using hs-GAL4 driver. Note normal development of all appendages. Eye in of the pharate adult in B is white because of prolonged preservation of the sample in ethanol prior to photography. (D) Partial rescue of tws^P/tws^P flies by en-GAL4 mediated UAS-Tws expression. Note that development of all appendages is partially rescued as Tws is expressed only in the posterior compartment. Overexpression of Tws in tws⁶⁰/tws⁶⁰ background induces gain-of-Wg phenotype. (E,F) Wild-type wing blades showing posterior hinge (E) and anterior margin (F) at higher magnification. (G) UAS-arm^S10; vg-GAL4 wing blade with tufts of ectopic bristles (arrow) at the posterior hinge. (H) 405-GAL4/UAS-arm^S10 wing blade with anterior margin-specific bristles in ectopic positions (arrow). Inset shows 405-GAL4 expression pattern. (I,J) Similar phenotypes (arrows) are observed when Tws is overexpressed in tws⁶⁰/tws⁶⁰ background using hs-GAL4.

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