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Fig. 7. Dominant-negative phenotypes caused by the overexpression of Tws in
wild-type background. (A-C and E) vg-GAL4/UAS-Tws wing discs stained
with Ct (A), Sca (B), Dll (C) and Vg (E) antibodies. (D) Wild-type wing disc
stained with anti-Vg antibodies. Tws overexpression caused the downregulation
of both short-range (Ct expression in the DV boundary and Sca expression in
the presumptive margin SMCs; arrow in B) and long-range (Dll and Vg) targets
of Wg signaling. Refer to Fig.
1L, Fig. 2C and
Fig. 2E, for wild-type
expression patterns of Ct, Sca and Dll, respectively. (F-G) Wild-type (F) and
vg-GAL4/UAS-Tws (G) wing discs stained with Arm. Note overexpression
of Tws causes reduction in cytoplasmic Arm levels. (H)
vg-GAL4/UAS-Tws wing blade showing serrated posterior margin. (I)
UAS-armS10; vg-GAL4/UAS-Tws wing blade. Note suppression
of Tws-induced serrated margin phenotype. This wing blade is indistinguishable
from UAS-armS10; vg-GAL4 wing blades (data not shown).
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