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Fig. 1. Early expression of Bmp receptors and Msx1 but not Msx3 repress neuronal
differentiation. (A-F) Immunofluorescence labeling with the antibody Tuj1 in
embryos electroporated at HH10-12 and assayed 48 hours post electroporation at
HH20-22. Electroporated constructs are indicated. Broken lines indicate the
midline of the neural tube. Horizontal lines indicate the width of the control
(left) versus the electroporated (right) side of the neural tube.
(A'-F') Immunofluorescence labeling with the Msx antibody showing
the endogenous Msx expression on the control side and the ectopic expression
of Msx on the electroporated side by Bmp signaling induction (A' and
B') or overexpression of Msx constructs (C'-F').
(G-J) Immunofluorescence labeling of neuronal differentiation markers Lhx2/9
(red) and Lhx1/5 (green) (G,H), and Isl1 (red) and Pax2 (green) (I,J) in
neural tubes electroporated with Msx1 or Msx1a. (K)
Schematic showing the dorsoventral boundaries of the ventricular zone markers
Msx, Cath1, chick Ngn1, Cash1, and the differentiation markers Lhx2/9, Lhx1/5,
Brn3a, Isl1, Pax2 and Lmx1b. dI1-6, dorsal interneuron populations 1-6; MN,
motor neuron; V0-1, ventral interneuron populations 0 and 1. In all panels
left is the control side and right is the electroporated side. Scale bar: 150
µm.
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