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Fig. 5. Roof-plate markers are induced by Bmp signaling and Msx1 but not Msx3. In situ hybridization of Bmp4 (A-E), Wnt1 (F-J) and immunofluorescence labeling of Lmx1 (K-O) on transverse sections of HH15-18 (A-C,F-H,K-M) or HH20-22 (D-E,IJ,N-O) neural tubes electroporated with ca-Bmpr1, Msx1 or Msx3 at stages indicated. Brackets indicate the size of each population on the control (left) and the electroporated (right) sides. Arrowheads indicate the midline in each panel. Insets in (A-E) show the expression of the GFP control plasmid on the electroporated side of the neural tube. (P) Quantification of Lmx+ cells/section on the control side and the electroporated side of the neural tubes shown in K-O. Data shown as mean±s.e.m., n=10 sections from at least four embryos; ***P<0.001. dI1,5, dorsal interneuron populations 1 or 5; FP, floor plate; hpe, hour post-electroporation; RP, roof plate. In all panels left is the control side and right is the electroporated side. Scale bar: 150 µm.





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