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Fig. 6. Msx3 induces neural progenitor cells to adopt a dorsal cell fate. (A-F)
Immunofluorescence labeling of Cath1 in HH20-22 neural tubes. Electroporation
of Msx3 in HH10-12 (A,B) or HH14-16 (D,E) embryos induces ventral
ectopic expression of Cath1 at HH20-22. (B,E) Higher magnification images of
the electroporated (right) side of the neural tubes in A and D showing Cath1
(red) and Msx (green) double positive cells (yellow, arrows) and
Cath1+Msx- cells (red, arrowheads). Overexpression of
Msx3a does not induce ectopic Cath1 expression (C,F). (G) Bar graph
indicating number of Cath1+ cells/section in Msx3-versus
Msx3a-electroporated embryos (late indicates electroporation at
HH14-16; early indicates electroporation at HH10-12) (mean±s.e.m.,
n=16 sections from at least four embryos;
***P<0.001, **P<0.005). In all
panels, left is the control side and right is the electroporated side. Scale
bars: 150 µm in A,C,D,F; 75 µm in B,E.
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