Figure 1
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Fig. S1. The germarial aMio signal is dependent on the
expression of the Mio antigen. Germaria were double-stained
with aMio (red)
and aOrb (green). (A) A mio1 mutant germarium contains no
detectable aMio
staining. Arrows indicate the oocyte. Staining in somatic cell membranes is background and
is also observed in wild type (see Fig. 6B). Part of UASp-mio (B,B¢) and UASp-mio;nanosGal4 germaria (C,C¢,D,D¢) showing early (arrowhead) and late region 2b
(arrow). B¢, C¢ and D¢ show only the aMio signal from B, C and D. Both germaria were photographed using identical
exposure times. Mio is overexpressed in C and
D, but not in B. Note that the aMio signal is brighter in the
oocyte nuclei in which Mio has been overexpressed.