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Fig. 1. Expression pattern of HtrA1. (A) Whole-mount in situ hybridization
of 12.5 dpc embryo. Embryos were hybridized with a sense probe (left), or with
an antisense probe (right). (B) In situ hybridization of a sagittal section of
12.5 dpc mouse embryo. HtrA1 expression (blue signals) is seen in the
vertebral column, hind limb, brain, gonad, heart, lung and abdominal skin. (C)
In situ hybridization of a sagittal section of 14.5 dpc embryo. In addition to
the sites mentioned in B, HtrA1 expression is seen in the
intervertebral disc, tracheal wall, cardiac outflow tracts and meninges. In
the hind limb, HtrA1 is expressed in the primordia of the extensor tendons.
(D) Magnified view of B showing the vertebral column and ribs. (E) Magnified
view of C showing the brain area near the fourth ventricle. Note that the
meninges also expressed HtrA1. (F) Immunostaining of a transverse section
through the heart at 11.5 dpc. HtrA1 protein (brown) is detected in the AV
cushion and outflow tract. (G) Magnified view of B showing the heart.
HtrA1 is expressed in the AV endocardial cushion. (H) Magnified view
of B showing the gonad. The Wolffian duct does not express HtrA1 but
the mesonephric tubules do. (I) Magnified view of B showing the lung. (J) In
situ hybridization of a section of 14.5 dpc maxillary skin. (K,L)
Immunostaining of the skin. The eyelid of an 18.5 dpc embryo (K) and the skin
from the back of a 9-day old pup (L) are shown. HtrA1 protein is detected in
the basal layer of epidermis (arrow), in the epithelial component of the
developing whisker (arrowhead). HtrA1 protein is also detected in the outer
root sheets of whiskers of the 9-day pup. a, cardiac atria; ao, aorta; as,
abdominal skin; av, atrioventricular cushion; b, bronchicles; c, condensing
cartilage; cp, choroid plexus; d, dermis; e, epidermis; et, primordium of
extensor tendon; g, gonad primordium; h, heart; hb, hind brain; hl, hind limb;
hs, hair shaft; id, intervertebral disc; in, intestine; irs, inner root
sheath; li, liver; lu, lung; M, Müllerian duct; m, meninges; mc,
metacephalon; me, mesonephric tubules; nt, neural tube; ot, outflow tract;
ors, outer root sheath; r, rib; t, tendon/ligament; tc, telencephalon; tr,
trachea; v, cardiac ventricle; ve, vertebral column; W, Wolffian duct; wf,
whisker follicle; 4v, 4th ventricle.
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