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Fig. 1. GA regulates stamen filament length via control of cell elongation. (A) SEMs of wild-type flowers at floral stages 10 and 13 are shown. Petals and stamens from the most advanced flower (middle bottom) in ga1-3 resembled the wild-type flower at stage 10 (top left). Stamen filament elongation is dramatically increased in ga1-3 plants lacking RGL1, RGL2 and RGA (Q2) and in ga1-3 plants lacking RGL1, RGL2, GAI and RGA (penta) compared with the wild type. Both Q2 and penta lines produced visible pollen grains. (B) Comparison of stamen and pistil lengths among different genotypes. Filament and pistil lengths were measured from SEM pictures (n=20). (C) SEM of stamen filament epidermal cells. ga1-3 stamen filaments have relatively short epidermal cells compared with those of wild type; cell length was restored in stamen filaments of ga1-3 plants lacking RGL1, RGL2, GAI and RGA (penta). Stamen filament segments shown were all from the middle part of the filament. (D) Average number of epidermal cells per stamen filament in wild type, ga1-3 and ga1-3 lacking RGL1, RGL2, GAI and RGA (penta).





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