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Fig. 6. Absence of RGL1, RGL2, GAI and RGA leads to GA-independent plant growth.
(A,B) Wild-type plants were compared with ga1-3 plants containing
loss-of-function mutations causing lack of various combinations of RGL1, RGL2,
GAI or RGA at 22 (A) and 48 (B) days. Note that ga1-3 plants lacking
RGL1, GAI and RGA or RGL2, GAI and RGA or all four DELLA proteins bolted
earlier than the wild-type control at 22 days. Genotypes are as indicated. (C)
Flowering time of various genotypes, measured as number of leaves at time of
flowering. In this case, ga1-3 plants under SD did not produce any
visible flower buds even though they had more than 50 leaves (marked with
*). Under LD condition, ga1-3 plants lacking RGL1, GAI and
RGA or RGL2, GAI and RGA or all four DELLA proteins all flowered with one or
two leaves less than did the wild-type control, although this small magnitude
of difference is hard to discern from the histogram shown here. (D) Plant
height of various genotypes at 28 days old under LD growth condition. Results
are presented as mean±standard error (n=30).
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