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Fig. 3. Ectopic expression of Tsh genes in Drosophila alters the
expression patterns of two tsh target genes. (A-C) Regulation of
wg expression by Tsh genes. (A) wg mRNA in a 12-13 stage
wild-type embryo showing absence of wg expression in the ventral
parts of the labial and maxillary segments (arrows). (B) Maintenance of
wg expression in the head segments by ectopic tsh and (C) in
69B>Tsh1 embryos (arrows, identical results with Tsh2 and
Tsh3). The maintenance of wg expression by tsh and
the three Tsh in the labial is completely penetrant. By contrast, the
maxillary segment is maintained in almost 75% of 69B>tsh
(n=212), 69B>Tsh1 (n=305) and
69B>Tsh3 (n=159), and in 
25% of 69B>Tsh2
(n=170). (D-G) Regulation of mod expression by Tsh genes.
(D) mod expression revealed by in situ staining in a stage 11
wild-type embryo. mod mRNA are detected in the labium (arrow). (E)
Reduced mod expression in the epidermis of the labial segment upon
ectopic expression of tsh (69B>tsh) or Tsh genes as
illustrated in F by Tsh1. Reduction of mod expression occurs
in roughly 75% of 69B>Tsh1 (n=291) and
69B>Tsh3 (n=170) and 50% of 69B>Tsh2
(n=230) embryos.
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