|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Tsh proteins are CtBP-dependent repressors. (A) Deletion of the
CtBP-interaction motif in Tsh abolishes the interaction with CtBP in vitro
(bottom) and fails to suppress mod expression in the labial segment
of 69B>tsh
PLDLS embryos (top, compare with
Fig. 3E). (B) pABgal-mouse
Tsh1, 2, and 3 (0.025 and 0.1 µg) alone repress pGL2-5xUAS-Luc reporter
activity (0.25 µg). (C) GST-pull down assay showing the interaction between
in vitro translated mouse Ctbp1 and GST-mouse Tsh fusion proteins.
Non-specific interaction is shown with GST alone. (D) In an independent
experiment from B, co-expression of 0.1 µg mouse Ctbp1 with 0.1 µg
Gal4-mouse Tsh fusions leads to a synergistic inhibition. Deletion of the
putative CtBP interaction motif in pABgal-mouse Tsh1 reduces the activity of
the reporter from 5 to 2.5-fold and abolishes the synergistic repression in
the presence of mouse Ctbp1. Mouse Ctbp1 alone does not affect the reporter
activity (not shown). Data are presented as fold repression relative to
luciferase activity, normalized to the ß-galactosidase activity, of the
reporter in presence of empty pABgal and pcDNA3.myc.
|
