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Fig. 8. Activation of the MAP Kinase is downstream of the Wnt/ß-Catenin pathway but does not requires intercellular communication. Immunolocalizations using an anti MAPK-P antibody on control embryos (A,D), zinc-treated (B), dnTCF RNA-injected (animalized) (E) or lithium treated (vegetalized) (C,F) embryos. Phenotypes of a control pluteus (G), dnTCF RNA injected animalized embryo (H) and a lithium vegetalized embryo (I). (J) Time course of MAPK activation analysed by western blot analysis and effect of cell dissociation. 16, sixteen-cell stage; 60, 60-cell stage; veB, very early blastula; eB, early blastula; phB, prehatching blastula; hB, hatching blastula; sB, swimming blastula; mB, mesenchyme blastula; lG, late gastrula; Pl, pluteus; vv, vegetal view. (K-M) Activation of ERK in dissociated blastomeres analysed by immunostaining. Note that the cellular aggregates visible on the pictures were not present during the culture but formed during fixation and the immunostaining protocol.





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