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Fig. 3. Overlapping functions of MP and NPH4 in embryos. (A) Two
upper panels (dark-field optics, top; schematic, bottom) illustrate phenotype
classes (from left): dicotyledonous with normal vasculature, dicotyledonous
with reduced secondary vein lobes, solitary midvein, fused cotyledons, single
cotyledons and club-shaped seedlings. Panel below shows the percentage of each
phenotype class in single and double mutants for nph4 in various
double homozygous allelic combinations with weak (mpG92; nph4-1),
intermediate (mpT370; nph4-1) and strong (mpG12; nph4-1)
mp alleles. Columns represent the proportion of each phenotype class
in % (broken lines indicate 25, 50, 75 and 100%). Approximately 100 double
mutants were evaluated for each genotype; 290 double mutants were evaluated
for mpG12; nph4-1. (B) Phenotype of a `club-shaped' seedling
(mpG12; nph4-1). Neither cotyledons nor any other lateral outgrowth
is visible and only very few disorganized vascular cells are being produced
(dark-field optics). (C) Leaf venation (xylem strands) in the first rosette
leaf (left) and first cauline leaf (right) of the genotypes: Col-0 wild type,
nph4-1 homozygous, 35S::MPAS in wild-type background and
35S::MPAS hemizygous in nph4-1 homozygous background. Note
that the venation pattern in nph4-1 single mutants is unaffected,
displaying primary (midvein), secondary (first order branches), tertiary and
some quarternary veins in the area enclosed by the secondary vein arches.
Tertiary and quarternary venation is strongly reduced in 35S::MPAS
plants. In 35S::MPAS; nph4-1 plants, tertiary and quarternary
venation is further reduced and the pattern of secondary vein lobes is
disrupted (dark-field optics).
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