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Fig. 5. RT-PCR analysis of JAG and JGL expression in different tissues. (A) Expression in wild type. Numbers refer to PCR cycles. RNA was extracted from roots of seedlings grown vertically on MS agar plates; vegetative shoots of 2-week-old plants; mature rosette leaves; mature cauline leaves; inflorescence stems; 4-week-old inflorescence shoots including flowers up to stage 12; and stage 13 open flowers. (B) JAG expression in inflorescences of jag mutants. Amplification of TUBULIN was used as a control.





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