|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Expression of JAG and FIL, examined by in situ
hybridization. (A-C, E-H) JAG expression in wild-type
Columbia. (A) Vegetative shoot apex from a 2-week-old plant. JAG is
expressed in incipient leaf primordia (ip) and older leaf primordia (lp), but
absent from the shoot apical meristem (vm) and petiole regions (brackets).
(B,C) Serial transverse sections of a vegetative shoot separated by 16 µm.
JAG is expressed in the leaf blade and is strongest in the marginal
regions (brackets) and excluded from the petiole. Asterisk marks same leaf in
both sections. Strong JAG expression is also seen in stipules (sp).
(D) FIL expression in wild-type Columbia inflorescence. Arrow
indicates the cryptic bract (cb). Inflorescence meristem (im), floral meristem
(fm). (E) JAG is expressed very early in the presumptive sepal (se)
primordia of late-stage 2 flowers. JAG expression is not detected in
floral or inflorescence meristems or the cryptic bract. (F) Stage 6 floral
primordia show strong expression in initiating stamens (st), carpels (ca) and
fading expression in sepals. (G) Transverse section of gynoecium. Floral stage
indicated in lower left corner. During stage 8, JAG is expressed in
the valves (v) and excluded from the replum (r). At stage 9, JAG
expression diminishes in the valves but is maintained near the valve margins.
(H) Expression of JAG during petal development from stage 8 to 11.
JAG accumulates in the distal region of petals and disappears by
stage 12. (I,J) JAG expression in jag-5D heterozygotes. (I)
Note weak signal throughout the inflorescence, with higher levels of
expression in the initiating bract (br) and inflorescence meristem. (J)
Prolonged and ectopic expression in stamen filament (fi), and gynoecium
including ovules (ov) in a stage 12 flower. (K) JAG is strongly
expressed in the ectopic bracts of ap1 mutants. Scale bars: 100 µm
(A-G, I, K), 50 µm (H), and 200 µm (J).
|
