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Fig. 5. Map-based cloning and sequence of JAG. (A) Schematic of positional cloning of the JAG locus showing the frequency of recombinant chromosomes identified in a mapping population with respect to molecular markers corresponding to BAC clones. Fine-scale mapping localized the JAG locus to the BAC T26J14 (black box). (B) Amino acid sequence of the JAG protein. Positions and nature of the jag-2 (asterisk) and jag-3 (black arrowhead) mutations are indicated. The EAR repression motif is boxed. A putative nuclear localization sequence is shown in bold font. The single C₂H₂-type zinc finger motif is underlined in black. A proline-rich motif is underlined in gray. (C) Amino acid alignment of the JAG C₂H₂ zinc finger domain with other Arabidopsis proteins. The positions of conserved cysteine and histidine residues involved in coordinating a zinc ion are indicated by asterisks. (D) Amino acid alignment of the JAG EAR repression motif with other Arabidopsis proteins. The EAR repression consensus motif is indicated below. Identical residues are in black boxes; similar residues in gray boxes.

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