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Fig. 6. Expression pattern of JAG. (A-L) RNA localization by in situ hybridization with JAG antisense probe. (A) Late transition stage embryo. (B) Heart stage embryo. (C) Torpedo stage embryo. (D) and (E) Consecutive longitudinal section through a seedling 3.5 days after germination. (F) Seedling 7 days after germination. (G) Longitudinal section of inflorescence meristem and early floral buds. (H) Transverse section of inflorescence meristem. (I) Expression in stage 3 floral bud. (J) Transverse section of stamen primordium of stage 6 flower. (K) Stage 8 petal primordium (arrowhead) longitudinal section. (L) Transverse section of stage 10 gynoecium. (M) Inflorescence of lfy-6 mutant with JAG expression in early bract primordia (arrows). (N) Confocal optical section showing the JAG::VENUS transgene expressed in seedling 4 days after germination. Nuclear expression of the fusion protein is confined to the growing region (arrowhead) of the leaf primordium. (O) JAG-VENUS subcellular localization (yellow) in cells of a leaf primordium. (P) Hoechst 33342 nuclear staining (blue). (Q) Merged image of O and P showing nuclear co-localization (green). Meristem is indicated by asterisk in some panels. Scale bar: 100 µm (G,K); 30 µm (O-Q); 50 µm in all other panels.





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