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Fig. 1. The GMC-1 division pattern in mitiP embryos. (A-F)
Embryos stained for Eve; (G,H) embryos stained for Eve (red) and Zfh1 (green;
yellow indicates co-localization); (I,J) embryos stained for Eve (red) and
22C10 (green). Embryos in A-H were analyzed by light microscopy, those in I
and J were analyzed by confocal microscopy. Anterior end is up, vertical lines
indicate the midline. Arrow indicates an RP2, small arrow indicates a sib.
(A,B) Wild-type embryos, note the smaller sib (with fading Eve expression) and
the larger RP2 (A); by 13 hours of age, the sib loses Eve expression and is no
longer visible (B). (C-F) mitiP embryos. Multi-cell
clusters are seen in the younger stage embryo (C, arrowheads), whereas in
older stage mitiP embryos (D-F) two to three RP2s were
observed per hemisegment. (E) Three RP2s and two sibs can be seen (sibs are
barely visible). (G) Wild-type embryo stained for Eve and Zfh1. Note that the
RP2 expresses Zfh1 at high levels. (H) mitiP embryo, all
three RP2s co-express Eve and Zfh1. (I,J) Wild-type (I) and
mitiP (J) embryos double stained for Eve and 22C10. The
two Eve-positive RP2s also express 22C10 and project normally.
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