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Fig. 3. Self-renewing asymmetric division of GMC-1 in nub gain-of-function
embryos. (A-D) Embryos stained for Eve; (E-J) embryos double stained for Eve
and Spectrin. Arrowhead indicates a GMC-1, arrow indicates an RP2, small arrow
indicates a sib. Thin, long arrows (E,G,J) denote indentations indicative of
the cell undergoing cytokinesis. (A) 
7.75-hour-old wild-type embryo. The
GMC-1 has divided to generate a sib and an RP2. (B) 9-hour-old wild-type
embryo. Note the fading Eve expression in sib. (C) 7.75-hour-old
UAS-nub; ftz-GAL4 embryo. The GMC-1 has divided to generate two
cells. (D) 9-hour-old UAS-nub; ftz-GAL4 embryo with a 3-cell
phenotype: two sibs and an RP2. (E,F) Wild-type embryos. Both nuclear division
and cytokinesis of GMC-1 is asymmetric (E; 7.5-hours old) and this gives
rise to the smaller sib and the larger RP2 (F; 8-hours old). (G)
7.5-hour-old UAS-nub; ftz-GAL4 embryo. The GMC-1 is undergoing a
nearly equal nuclear division and cytokinesis to self-renew and to generate an
RP2. (H) 8.5-hourold UAS-nub; ftz-GAL4 embryo. The GMC-1 is in
the process of dividing to generate a smaller sib; an Eve-positive RP2 has
already been generated in this hemisegment (the top cell). (I,J) Two different
focal planes of the same area of an 8.5-hour-old UAS-nub;
ftz-GAL4 embryo, showing a sib cell generated earlier (I) and the GMC-1
dividing to generate two RP2s (J). The long arrow marks the position of the
sib. Scale bars: 7.5µm for A-D; 5µm for E-J.
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