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Fig. 7. Self-renewing asymmetric division in ago mutant embryos. (A-C)
Embryos stained for Eve. (D-I) Merged images of embryos stained for Eve (red)
and CycE (green). Arrow indicates an RP2, small arrow indicates a sib,
arrowhead indicates a GMC-1. Vertical lines mark the midline. (A) An
9.5-hour-old ago mutant embryo. The hemisegment on the left has
the 3-cell phenotype (two sibs and one RP2). (B,C) An 11-hour-old
ago mutant embryo with the same segment shown in two different focal
planes. The hemisegment on the left has the 3-cell phenotype, two RP2s (B) and
one sib with fading Eve expression (C). (D,E) A late GMC-1 in an ago
mutant embryo (7.25 hours old) with high levels of CycE protein. (F,G) An
7.75-hour-old ago mutant embryo in which the GMC-1 appears to
have nearly completed its division to generate two cells of more or less equal
sizes. One of these two cells, presumably the self-renewed GMC-1, has high
levels of CycE and the other cell, presumably the RP2, has very low levels of
CycE. (H,I) A hemisegment showing a 3-cell phenotype in an 9-hour-old
ago mutant embryo. All three cells have undetectable levels of CycE.
Scale bars: 10µm for A-C; 7.5µm for D-I.
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