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Fig. 8. The cell fate of the differentiating progeny of a GMC-1 self-renewing asymmetric division is Insc and Nb dependent. (B,D,F) Eve is red, Zfh1 is green; (J-O) Eve is green and Insc is red. Anterior end is up, vertical lines mark the midline. In wild type, there is only one Eve- (A) and Eve- and Zfh1 (B)-positive RP2 per hemisegment, whereas in insc mutants, the RP2 is duplicated, both being Eve- (B) and Zfh1 (D)-positive. (E,F) In miti^P; insc embryos, the GMC-1 generates an RP2 in each of its self-renewing divisions (the right hemisegments). (G) Wild-type embryo with an RP2 and a sib. (H) nb mutant. The GMC-1 has symmetrically divided to generate two sibs. (I) miti^P; nb embryo. In the right hemisegment the sib cells have lost Eve expression (arrowhead), whereas in the left hemisegment several sib cells can be still seen. (J-O) Localization of Insc in GMC-1 is affected in miti^P embryos. Only the merged images are shown. Insc is asymmetrically distributed in wild-type GMC-1 (J), but its distribution is non-asymmetric in miti^P embryos (K). Localization of Insc is also affected in several other unidentified GMCs (e.g. a row 4 GMC; L). Localization of Insc is not affected in GMC1-1a (N; a wild type GMC1-1a is shown in M), or several other unidentified Miti/Nub-negative GMCs (O). Scale bars: 15 µm for A-I; 10 µm for J-O.

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