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Fig. 9. GMCs for the RP2/sib, U, CQ and EL neuronal lineages do not undergo
additional rounds of cell division in prospero mutants. Embryos are
single stained for Eve (A-D) and double stained for Eve (red) and Zfh1 (green)
(E-J; only one half-segment is shown). Midline is marked by vertical lines.
Embryos mutant for pros have holes in the midline and a mis-routed
axon scaffold, thus the neuronal lineages are farther apart. The loss of RP2,
U and CQ lineages is partially penetrant in this mutant allele (see text for
details). (A) 
10-hour-old wild-type embryo. The number of U neurons is
usually four in wild type, CQs are hidden beneath the U cluster, but are
visible in the hemisegment on the left. (B) 13-hour-old wild-type embryo.
Us and CQs are out of the focal plane. ELs are also slightly out of the focal
plane. (C) 10-hourold pros mutant embryo. An RP2 and a sib are
shown. aCC/pCC, Us and CQs are missing in this segment. (D) 13-hour-old
pros mutant embryo. An RP2 is flanked by two Us on top and three Us
on the bottom. (E) 13-hour-old wild type, note that RP2 and aCC are Eve
and Zfh1 positive. (F) 13-hour-old pros mutant. Both aCC and pCC
are missing (marked by a star). (G) 13-hour-old wild-type embryo. Out of
four Us, two are Eve and Zfh1 positive. The other two are only Eve positive.
(H) 13-hour-old pros mutant embryo. There are only three U
neurons, one of the two Eve- and Zfh1-positive Us is missing. (I)
13-hour-old wild-type embryo. In wild type there are three CQs, all are
Eve and Zfh1 positive (one of the CQs in this panel is out of the focal
plane). The number of ELs varies between eight and ten in the abdominal
segments. (J) 13-hour-old pros mutant embryo. There is only one
CQ in this hemisegment. The number of ELs is usually the same as in wild type
(some of the ELs are out of the focal plane in this panel).
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