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Files in this Data Supplement:
Fig. S1. Tracks of migrating AVE cells. (A) Tracks of five cells from Movie 3B, overlaid on the last frame of the movie. The cell tracks are constructed from 11 time points and are therefore comparatively short. Cell 1 and cell 2 start to move laterally at the junction of the epiblast with the extra-embryonic ectoderm. (B) Tracks of four cells from Movie 4A, overlaid on the last frame of the movie. Note that cell 2 starts out to the left of cell 3, but by the time they reach the extra-embryonic ectoderm they have exchanged positions, so that cell 2 is now on the right.
Movie 1. Time-lapse movie of a cultured 5.5 dpc embryo. The embryo is oriented with the presumptive anterior to the left. EGFP fluorescence (green) marks the AVE, which moves unidirectionally to the extra-embryonic ectoderm in approximately 4 hours, but does not move beyond it. The embryo was imaged at 15 minute intervals with phase contrast and fluorescence optics.
Movie 2. Time-lapse movie of a cultured 5.5 dpc embryo, showing the migration of AVE cells. The embryo is oriented with the presumptive anterior facing the viewer. EGFP-expressing AVE cells move proximally from the distal tip of the embryo until they reach the extra-embryonic ectoderm and then start spreading laterally. Cells cover this distance in about 5 hours and project filopodia in the direction of motion. The embryo was imaged at 12 minute intervals. The tracks of several migrating cells from this embryo are shown in Fig. 4 of the accompanying paper.
Movie 3. Time-lapse movie showing just the fluorescence channel of Movie 2, so that EGFP-expressing AVE cells can be seen more clearly. Note that fluorescent cells are interspersed with non-fluorescent cells in the AVE.
Movie 4. Time-lapse movie of a cultured 5.5 dpc embryo, showing the migration of EGFP-expressing AVE cells. The embryo is oriented with the presumptive anterior side facing the viewer, but twisted slightly to the left. AVE cells move proximally until they reach the extra-embryonic ectoderm and then start spreading laterally. The embryo was imaged at 15 minute intervals. The tracks of several migrating cells from this embryo are shown in the Fig. S1 at http://dev.biologists.org/supplemental/.
Movie 5. Time-lapse movie showing just the fluorescence channel of Movie 4, so that EGFP-expressing AVE cells can be seen more clearly.
Movie 6. Time-lapse movie of a cultured 5.5 dpc embryo, showing the migration of EGFP-expressing AVE cells. The embryo is oriented with the presumptive anterior side facing the viewer, but twisted slightly to the left. AVE cells move proximally until they reach the extra-embryonic ectoderm and then start spreading laterally. The embryo was imaged at 10 minute intervals. The tracks of several migrating cells from this embryo are shown in the Fig. S1 at http://dev.biologists.org/supplemental/.
Movie 7. Time-lapse movie showing just the fluorescence channel of movie 6, so that the EGFP-expressing AVE cells can be seen more clearly.
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