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Fig. 2. Frontal view (A-K) of a developing 5.5 dpc embryo (see Movies 2 and 3). (L) Drawing depicting the orientation of the embryo, with anterior facing the reader. The embryo was imaged every 12 minutes with phase-contrast and fluorescence optics. In this figure the phase-contrast images have been made darker so that the fluorescent AVE cells can be seen more clearly. Time from the start of imaging is indicated in hours and minutes at the bottom right of each frame. AVE cells move proximally until they reach the junction of the epiblast with the extra-embryonic ectoderm and then start spreading laterally. Cells cover this distance in about 5 hours and project filopodia in the direction of motion (see Fig. 3). Note that fluorescent cells can be seen interspersed with non-fluorescent cells at time points later than 2 hours. Scale bar in A: 50 µm for A-L.





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