DEV01024 Supplemental Figure

Files in this Data Supplement:

• Figure 1 -

  Fig. S1. Disruption of Nkx6.1 by a morpholino-modified antisense oligonucleotide. (A) The genomic organization of chick Nkx6.1 surrounding the homeodomain (HD). Exons are shown in blue and introns are indicated by black lines, their size is shown in basepairs (bp). The morpholino (MO) targets the splice acceptor site in the intron preceding the HD. Arrows indicate the placing of the primers used in the RT-PCR. (B) Ethidium bromide-stained agarose gel of a RT-PCR of rostral hindbrain tissue 2-3 days after electroporation with either the control morpholino (cMO) or the morpholino against Nkx6.1 (6.1 MO). Large parts of the tissue do not take up the morpholino, and thus the wild-type transcript is readily amplified in both the control (cMO) and 6.1 MO +RT reactions. A larger amplification product (arrow) is obtained only in the 6.1 MO +RT reaction, which represents retention of the 100 bp intron.