spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Ectopic generation of 5-HT neurons by Gata2 in r1. (A,B) Whole-mount immunofluorescence of chick hindbrain 4 days after electroporation of Gata2, with antibodies against 5-HT (red, A,B) and 3C2, a viral envelope marker (B, green). Endogenous 5-HT neurons (en.) versus ectopic 5-HT neurons (ect.) are bracketed (A). An arrowhead indicates the lack of 5-HT neurons in the midbrain (MB) despite the presence of exogenous Gata2 (B, green). The midbrain (MB) and mid-hindbrain boundary (MHB, arrow) are marked. (C) Immunofluorescence for 5-HT on thin (10-12 µm) sections through r1 of the hindbrain. The dorsal roof plate (rp) and ventral floor plate (fp) are marked; endogenous 5-HT neurons (en. 5-HT) versus ectopic 5-HT neurons (ect. 5-HT), and unelectroporated side (control side) are marked by brackets. (D-G) Higher magnification of ectopic 5-HT positive neurons (red; D,E,G) from C, showing cell bodies and processes (D, arrows) and co-localization (E-G, arrows) with 3C2 (F,G, green). Scale bars: C, 75 µm; D, 12.5 µm; G, 8 µm.





Right arrow Return to article